ABSTRACT
Objective To observe the sealing effect of transient pinus massoniana bark extract (PMBE)pre-coating on dentin tubules and its wear resistance so as to provide an experimental basis for PMBE to be used to prevent and treat dentin hypersensitivity.Methods The model of dentin hypersensitivity was established by acid etching method.The samples were transient pre-coating with 80 g/L of PMBE ethanol solution,and then some of them experienced brushing wear treatment with ethanol group and fluorine vanish group as negative and positive controls.The surface and profile morphologies of the samples were observed using field emission scanning electron microscope (FESEM)in each group.The area of dentin tubular openings (ADTO)and percentage area of dentin tubular openings (PADTO)were also measured.Results The dentin tubules were opened completely and the lumen of dentin tubules was empty in ethanol group.The dentin tubules were completely or basically closed and some films or plugging could be seen in the lumen of dentin tubules in fluorine vanish/immediate group and PMBE/immediate group.The dentin tubules were partly opened and some wear marks could be seen on the dentin surface in fluorine vanish/wear groups,PMBE/wear groups.ADTO and PADTO in ethanol group were significantly higher than those in fluorine vanish groups and PMBE groups (P＜0.05).ADTO and PADTO did not significantly differ between fluorine vanish/wear groups and PMBE/wear groups (P＞0.05),which were all significantly higher than those in fluorine vanish/immediate group and PMBE/immediate group (P＜0.05).Conclusion Local transient pre-coating of PMBE ethanol solution can achieve fine immediate sealing effect of dentin tubules and some degree of wear resistance.
ABSTRACT
Objective:To evaluate the effect of pinus massoniana bark extract (PMBE) and grape seed extract (GSE) on dentin demineralization caused by acid.Methods:40 root dentin blocks with half of the surface covered were randondy divided into 4 groups (n =10).All samples were subjected to pH cycling for 8 days,and deionized distilled water(DDW),0.1％NaF,12％ PMBE solution and 12％ GSE solution were used as the experimental solutions in the 4 groups.The dentin mineral density(DMD) of the both sides was determined using micro-computed tomography.The D-value of DMD between nn-demineralized and demineralized side (△DMD)was calculated.The samples were observed with field emission scanning electron microscops (FE-SEM).Results:The △DMD of DDW,NaF,PMBE and GSE groups was 198.64 ±59.97,45.94 ±24.21,90.23 ±28.77 and 105.07 ±29.53 respectively.The △DMD between PMBE and GSE groups had no significant difference (P ＞ 0.05),which were both higher than that of NaF group (P ＜ 0.05) and lower than that of DDW group(P ＜ 0.05).The FE-SEM revealed that the dentin tubules in DDW group were completely open,but in NaF group were essentially closed in PMBE group and GSE group were spindle shaped or narrow crack opening.Conclusion:PMBE and GSE had almost the same effect on improving the acid resistance of dentin.